sRNA Delivery

siRNA Delivery AffiNANOTECH  refers to the application of affinity-functionalized nanotechnology platforms to deliver small interfering RNA (siRNA) molecules specifically to target cells or tissues. By combining advanced nanoparticle engineering with affinity-based targeting strategies, this approach aims to improve the stability, specificity, and efficiency of siRNA delivery, thereby enhancing gene silencing outcomes while minimizing off-target effects and systemic toxicity.

Technical Content and Mechanism

• Nanocarrier Design for siRNA Delivery:
  • Material Selection:
    Nanocarriers for siRNA are often composed of biodegradable polymers (e.g., PLGA, chitosan), lipid-based formulations (liposomes, lipid nanoparticles [LNPs]), or inorganic materials (e.g., gold, silica).
• siRNA Loading and Protection:
  • Complexation or Encapsulation:
    siRNA molecules are either electrostatically complexed with cationic components of the nanoparticle or encapsulated within the nanoparticle core. This shields siRNA from nucleases, prevents premature degradation, and enhances systemic stability.
• Targeting, Cellular Uptake, and Endosomal Escape:
  • Selective Binding to Target Cells:
    Affinity ligands direct the siRNA-loaded nanocarriers to cells expressing the target receptors. This receptor-mediated binding increases local nanoparticle concentration at the target site and facilitates uptake.

Applications

• Targeted Gene Silencing:
  Affinity-targeted siRNA delivery systems can selectively knock down disease-related genes in cancer cells, inflammatory cells, or other pathological cell types, offering potential treatments for cancers, genetic disorders, and autoimmune diseases.
• Precision Medicine:
  By modifying affinity ligands to target patient-specific biomarkers, siRNA nanocarriers can provide personalized therapeutic interventions that downregulate aberrant gene expression with high specificity.
• Combination Therapies:
  siRNA delivery platforms can be combined with chemotherapeutics or other biological agents in a single nanoparticle to achieve synergistic effects, such as sensitizing tumor cells to drugs or modulating the tumor microenvironment.

siRNA Delivery AffiNANOTECH harnesses the specificity of affinity ligands and the protective, efficient delivery capabilities of advanced nanocarriers to transport siRNA molecules to designated cells.